Abstract's Details

Nanoparticle Diffractive Imaging Using Free-electron Lasers
Abstract ID	UMTG:9-3
Presenter	Sebastien Boutet
Presentation Type	SSRL/LCLS
Full Author List	S. Boutet (1), M. J. Bogan (2), U. Rohner (2), A. Barty (2), W. H. Benner (2), M. Frank (2), M. M. Seibert (3), J. Hajdu (3), S. Marchesini (4), H. N. Chapman (2)
Affiliations	(1) Stanford Linear Accelerator Center (2) Lawrence Livermore National Laboratory (3) Uppsala University (4) Lawrence Berkeley National Laboratory
Category	Instrumentation/Development
Abstract	Using the extremely short and intense X-ray pulses that will be produced by the LCLS, it should be possible to record diffraction patterns from single biomolecules beyond the classical radiation damage limit. The X-ray pulse duration can be made shorter than the dynamics involved in the damage process allowing for a diffraction pattern of an undamaged sample to be measured. Such experiments will require new technologies to be developed. Experiments were carried out at the Free Electron LASer in Hamburg (FLASH) in order to develop these new technologies. For example, a containerless delivery of the sample to the FEL beam was constructed to eliminate scattering from a sample support. I will discuss experiments that have demonstrated the concept of flash diffractive imaging in which the sample is destroyed by the beam but not before an interpretable diffraction pattern can be measured. I will also discuss results demonstrating single-particle injection into the FEL beam. Diffraction patterns of single particles in the gas phase illuminated by the coherent beam were measured and images were reconstructed using phase retrieval techniques. Finally, I will briefly discuss the Coherent X-ray Imaging (CXI) instrument being built for single-particle imaging at LCLS.
Footnotes
Funding Acknowledgement

Possible Imaging Science with LCLS
Abstract ID	W:IMG-03
Presenter	Sebastien Boutet
Presentation Type	Imaging Workshop
Full Author List	S. Boutet (1)
Affiliations	(1) Stanford Linear Accelerator Center
Category	Instrumentation/Development
Abstract	The Coherent X-ray Imaging (CXI) instrument, a hard X-ray imaging beamline is currently being built to utilize the unique LCLS beam. This instrument will be primarily dedicated to single nano-particle diffractive imaging at near atomic resolution. It is however being designed as a very versatile instrument allowing for a host of hard x-ray imaging experiments to be performed. The concepts behind the instrument will be discussed. Also, the design and unique capabilities of the CXI instrument will be introduced. Finally, some new science that will be uniquely achievable with this instrument will be discussed.
Footnotes
Funding Acknowledgement

Coherent X-ray Imaging Instrument at the LCLS
Abstract ID	I&D-01
Presenter	Sebastien Boutet
Presentation Type	Poster
Full Author List	Sebastien Boutet (1)
Affiliations	(1) LUSI/LCLS, SLAC
Category	Instrumentation/Development
Abstract	The ultrashort intense coherent hard X-ray pulses that will be produced by the LCLS will allow for the diffraction pattern of a single object of size ranging between 1 micron and a 10 nm to be measured with a single pulse. In the case of biological molecules, this will allow the classical damage limit, stating that the photons needed for the measurement at a certain resolution also destroy the features of interest, to be overcome by having all the photons interact with the sample before the relevant damage processes can occur. The Coherent X-ray Imaging (CXI) instrument built by the LUSI project at LCLS will be used to measure single pulse diffraction patterns of any nanoparticle of interest injected directly into the LCLS beam, without a support, using a single particle injector. The CXI instrument is designed for the ultimate goal of 3D atomic structure determination of reproducible biomolecules. The poster will describe the coherent imaging technique as well as introduce the main components of the CXI instrument.
Footnotes
Funding Acknowledgement